2.43
1,65
1,98
0,73
1,88
1.81
2.43
2.2 Zat standar yang digunakan dalam kurva kalibrasi distribusi massa molekul relatif: insulin, mikopeptida, glisin-glisin-tirosin-arginin, glisin-glisin-glisin
3 Instrumen dan peralatan
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27,5
Secara keseluruhan, proporsi asam amino dalam produk Sustar lebih tinggi daripada dalam produk Zinpro.
Bagian 8 Dampak penggunaan
Pengaruh berbagai sumber mineral mikro terhadap kinerja produksi dan kualitas telur ayam petelur pada periode akhir bertelur.
Proses Produksi
Teknologi khelasi tertarget
Teknologi emulsifikasi geser
Teknologi penyemprotan dan pengeringan bertekanan
Teknologi pendinginan & pengeringan udara
Teknologi pengendalian lingkungan tingkat lanjut
Lampiran A: Metode untuk Penentuan distribusi massa molekul relatif peptida
Penerapan standar: GB/T 22492-2008
1. Prinsip Pengujian:
Hal ini ditentukan dengan kromatografi filtrasi gel kinerja tinggi. Artinya, dengan menggunakan pengisi berpori sebagai fase diam, berdasarkan perbedaan ukuran massa molekul relatif komponen sampel untuk pemisahan, yang dideteksi pada ikatan peptida dengan panjang gelombang serapan ultraviolet 220 nm, menggunakan perangkat lunak pengolahan data khusus untuk penentuan distribusi massa molekul relatif dengan kromatografi filtrasi gel (yaitu, perangkat lunak GPC), kromatogram dan datanya diproses, dihitung untuk mendapatkan ukuran massa molekul relatif peptida kedelai dan rentang distribusinya.
2. Reagen
Air yang digunakan dalam percobaan harus memenuhi spesifikasi air sekunder dalam GB/T6682, dan reagen yang digunakan, kecuali untuk ketentuan khusus, harus murni secara analitis.
2.1 Reagen meliputi asetonitril (murni secara kromatografi), asam trifluoroasetat (murni secara kromatografi),
2.2 Zat standar yang digunakan dalam kurva kalibrasi distribusi massa molekul relatif: insulin, mikopeptida, glisin-glisin-tirosin-arginin, glisin-glisin-glisin
3 Instrumen dan peralatan
3.1 Kromatograf Cair Kinerja Tinggi (HPLC): stasiun kerja kromatografi atau integrator dengan detektor UV dan perangkat lunak pengolahan data GPC.
3.2 Unit filtrasi vakum dan penghilangan gas fase bergerak.
3.3 Timbangan elektronik: nilai skala 0,000 1g.
4 Langkah Operasional
4.1 Kondisi kromatografi dan percobaan adaptasi sistem (kondisi referensi)
- 4.1.1 Kolom kromatografi: TSKgelG2000swxl300 mm×7,8 mm (diameter dalam) atau kolom gel lain dengan tipe yang sama dan kinerja serupa yang sesuai untuk penentuan protein dan peptida.
- 4.1.2 Fase gerak: Asetonitril + air + asam trifluoroasetat = 20 + 80 + 0,1.
- 4.1.3 Panjang gelombang deteksi: 220 nm.
- 4.1.4 Laju alir: 0,5 mL/menit.
- 4.1.5 Waktu deteksi: 30 menit.
- 4.1.6 Volume injeksi sampel: 20μL.
- 4.1.7 Suhu kolom: suhu ruangan.
- 4.1.8 Untuk memastikan sistem kromatografi memenuhi persyaratan deteksi, ditetapkan bahwa dalam kondisi kromatografi di atas, efisiensi kolom kromatografi gel, yaitu jumlah pelat teoritis (N), tidak kurang dari 10.000 yang dihitung berdasarkan puncak standar tripeptida (Glisin-Glisin-Glisin).
- 4.2 Pembuatan kurva standar massa molekul relatif
- Larutan standar peptida dengan massa molekul relatif berbeda di atas dengan konsentrasi massa 1 mg/mL disiapkan dengan pencocokan fase gerak, dicampur dalam proporsi tertentu, kemudian disaring melalui membran fase organik dengan ukuran pori 0,2 μm~0,5 μm dan diinjeksikan ke dalam sampel, lalu kromatogram standar diperoleh. Kurva kalibrasi massa molekul relatif dan persamaannya diperoleh dengan memplot logaritma massa molekul relatif terhadap waktu retensi atau dengan regresi linier.
4.3 Perlakuan sampel
Timbang secara akurat 10 mg sampel dalam labu volumetrik 10 mL, tambahkan sedikit fase gerak, kocok dengan ultrasonik selama 10 menit, sehingga sampel larut dan tercampur sempurna, encerkan dengan fase gerak hingga mencapai skala yang ditentukan, lalu saring melalui membran fase organik dengan ukuran pori 0,2 μm~0,5 μm, dan filtrat dianalisis sesuai dengan kondisi kromatografi pada A.4.1.
- 5. Perhitungan distribusi massa molekul relatif
- Setelah menganalisis larutan sampel yang disiapkan pada 4.3 di bawah kondisi kromatografi 4.1, massa molekul relatif sampel dan rentang distribusinya dapat diperoleh dengan mensubstitusikan data kromatografi sampel ke dalam kurva kalibrasi 4.2 dengan perangkat lunak pengolahan data GPC. Distribusi massa molekul relatif dari peptida yang berbeda dapat dihitung dengan metode normalisasi luas puncak, sesuai dengan rumus: X=A/A total×100
- Dalam rumus: X - Fraksi massa peptida dengan massa molekul relatif dalam total peptida dalam sampel, %;
- A - Luas puncak peptida dengan massa molekul relatif;
- Total A - jumlah luas puncak dari setiap peptida massa molekul relatif, dihitung hingga satu angka desimal.
- 6. Pengulangan
- Selisih absolut antara dua penentuan independen yang diperoleh dalam kondisi pengulangan tidak boleh melebihi 15% dari rata-rata aritmatika kedua penentuan tersebut.
- Lampiran B: Metode Penentuan Asam Amino Bebas
- Penerapan standar: Q/320205 KAVN05-2016
- 1.2 Reagen dan bahan
- Asam asetat glasial: murni secara analitis
- Asam perklorat: 0,0500 mol/L
- Indikator: Indikator kristal violet 0,1% (asam asetat glasial)
- 2. Penentuan asam amino bebas
Sampel dikeringkan pada suhu 80°C selama 1 jam.
Tempatkan sampel dalam wadah kering agar dingin secara alami hingga suhu ruangan atau dinginkan hingga suhu yang dapat digunakan.Timbang sekitar 0,1 g sampel (akurasi hingga 0,001 g) ke dalam labu erlenmeyer kering 250 mL.Segera lanjutkan ke langkah berikutnya untuk mencegah sampel menyerap kelembapan lingkungan sekitar.Tambahkan 25 mL asam asetat glasial dan aduk rata selama tidak lebih dari 5 menit.Tambahkan 2 tetes indikator kristal violet.Lakukan titrasi dengan larutan titrasi standar asam perklorat 0,0500 mol/L (±0,001) hingga larutan berubah warna dari ungu menjadi titik akhir.
Catat volume larutan standar yang digunakan.
- Lakukan uji coba kosong pada waktu yang bersamaan.
- 3. Perhitungan dan hasil
- Kandungan asam amino bebas X dalam reagen dinyatakan sebagai fraksi massa (%) dan dihitung menurut rumus: X = C × (V1-V0) × 0,1445/M × 100%, dalam rumus:
- C - Konsentrasi larutan asam perklorat standar dalam mol per liter (mol/L)
- V1 - Volume yang digunakan untuk titrasi sampel dengan larutan asam perklorat standar, dalam mililiter (mL).
- Vo - Volume yang digunakan untuk blanko titrasi dengan larutan asam perklorat standar, dalam mililiter (mL);
M - Massa sampel, dalam gram (g).
| 0,1445: Massa rata-rata asam amino yang setara dengan 1,00 mL larutan asam perklorat standar [c (HClO4) = 1,000 mol / L]. | 4.2.3 Larutan titrasi standar serium sulfat: konsentrasi c [Ce (SO4) 2] = 0,1 mol/L, disiapkan sesuai dengan GB/T601. | |
| Penerapan standar: Q/70920556 71-2024 | 1. Prinsip determinasi (Fe sebagai contoh) | Kompleks besi asam amino memiliki kelarutan yang sangat rendah dalam etanol anhidrat, sedangkan ion logam bebas larut dalam etanol anhidrat. Perbedaan kelarutan antara keduanya dalam etanol anhidrat dimanfaatkan untuk menentukan laju khelasi kompleks besi asam amino. |
| Dalam rumus: V1 - volume larutan standar serium sulfat yang digunakan untuk titrasi larutan uji, mL; | Etanol anhidrat; sisanya sama dengan klausul 4.5.2 dalam GB/T 27983-2011. | 3. Langkah-langkah analisis |
| Lakukan dua percobaan secara paralel. Timbang 0,1 g sampel yang telah dikeringkan pada suhu 103±2℃ selama 1 jam, dengan ketelitian 0,0001 g, tambahkan 100 mL etanol anhidrat untuk melarutkan, saring, residu hasil penyaringan dicuci dengan 100 mL etanol anhidrat setidaknya tiga kali, kemudian pindahkan residu ke dalam labu Erlenmeyer 250 mL, tambahkan 10 mL larutan asam sulfat sesuai dengan klausul 4.5.3 dalam GB/T27983-2011, dan kemudian lakukan langkah-langkah berikut sesuai dengan klausul 4.5.3 “Panaskan untuk melarutkan dan kemudian dinginkan” dalam GB/T27983-2011. Lakukan uji blanko secara bersamaan. | 4. Penentuan kandungan zat besi total | 4.1 Prinsip penentuannya sama dengan klausul 4.4.1 dalam GB/T 21996-2008. |
4.2. Reagen & Larutan
| 4.2.1 Asam campuran: Tambahkan 150 mL asam sulfat dan 150 mL asam fosfat ke dalam 700 mL air dan aduk hingga rata. | 4.2.2 Larutan indikator natrium difenilamina sulfonat: 5 g/L, disiapkan sesuai dengan GB/T603. | 4.2.3 Larutan titrasi standar serium sulfat: konsentrasi c [Ce (SO4) 2] = 0,1 mol/L, disiapkan sesuai dengan GB/T601. | |
| 4.3 Langkah-langkah analisis | Lakukan dua percobaan secara paralel. Timbang 0,1 g sampel, dengan ketelitian 0,20001 g, masukkan ke dalam labu Erlenmeyer 250 mL, tambahkan 10 mL asam campuran, setelah larut, tambahkan 30 mL air dan 4 tetes larutan indikator natrium dianilin sulfonat, kemudian lakukan langkah-langkah berikut sesuai dengan klausul 4.4.2 dalam GB/T21996-2008. Lakukan uji blanko secara bersamaan. | 4.4 Penyajian hasil | Kandungan besi total X1 dari kompleks besi asam amino dalam hal fraksi massa besi, nilai yang dinyatakan dalam %, dihitung menurut rumus (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - larutan standar serium sulfat yang dikonsumsi untuk titrasi larutan blanko, mL; | V0 - larutan standar serium sulfat yang dikonsumsi untuk titrasi larutan blanko, mL; | C - Konsentrasi sebenarnya dari larutan standar serium sulfat, mol/L5. Perhitungan kandungan besi dalam khelatKandungan besi X2 dalam khelat dalam bentuk fraksi massa besi, nilai yang dinyatakan dalam %, dihitung menurut rumus: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| Dalam rumus: V1 - volume larutan standar serium sulfat yang digunakan untuk titrasi larutan uji, mL; | V2 - larutan standar serium sulfat yang digunakan untuk titrasi larutan blanko, mL;nom1-Massa sampel, g. Ambil rata-rata aritmatika dari hasil penentuan paralel sebagai hasil penentuan, dan perbedaan absolut dari hasil penentuan paralel tidak lebih dari 0,3%. | 0,05585 - massa besi(II) yang dinyatakan dalam gram setara dengan 1,00 mL larutan standar serium sulfat C[Ce(SO4)2.4H20] = 1,000 mol/L.nom1-Massa sampel, g. Ambil rata-rata aritmatika dari hasil penentuan paralel sebagai hasil penentuan, dan perbedaan absolut dari hasil penentuan paralel tidak lebih dari 0,3%. | 6. Perhitungan laju khelasiTingkat khelasi X3, nilai yang dinyatakan dalam %, X3 = X2/X1 × 100Lampiran C: Metode untuk Penentuan Tingkat Khelasi Zinpro |
Penerapan standar: Q/320205 KAVNO7-2016
1. Reagen dan bahan
a) Asam asetat glasial: murni secara analitis; b) Asam perklorat: 0,0500 mol/L; c) Indikator: indikator kristal violet 0,1% (asam asetat glasial)
2. Penentuan asam amino bebas
2.1 Sampel dikeringkan pada suhu 80°C selama 1 jam.
2.2 Tempatkan sampel dalam wadah kering agar dingin secara alami hingga suhu ruangan atau dinginkan hingga suhu yang dapat digunakan.
2.3 Timbang sekitar 0,1 g sampel (akurasi hingga 0,001 g) ke dalam labu erlenmeyer kering 250 mL.
2.4 Segera lanjutkan ke langkah berikutnya untuk mencegah sampel menyerap kelembapan lingkungan.
2.5 Tambahkan 25 mL asam asetat glasial dan aduk rata selama tidak lebih dari 5 menit.
2.6 Tambahkan 2 tetes indikator kristal violet.
2.7 Titrasi dengan larutan titrasi standar asam perklorat 0,0500 mol/L (±0,001) hingga larutan berubah warna dari ungu menjadi hijau selama 15 detik tanpa perubahan warna sebagai titik akhir.
2.8 Catat volume larutan standar yang digunakan.
2.9 Lakukan uji blanko secara bersamaan.
- 3. Perhitungan dan hasil
- Catalan
- Physicochemical parameters
V1 - Volume yang digunakan untuk titrasi sampel dengan larutan asam perklorat standar, dalam mililiter (mL).
Vo - Volume yang digunakan untuk blanko titrasi dengan larutan asam perklorat standar, dalam mililiter (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Alamat: No. 147 Jalan Qingpu, Kota Shouan, Kabupaten Pujiang, Kota Chengdu, Provinsi Sichuan, Tiongkok
Telepon: 86-18880477902
Produk
Mineral jejak anorganik
- Mineral mikro organik
- Bahasa Swahili
- Layanan yang disesuaikan
- Tautan cepat
Profil Perusahaan
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Bahasa Gujarati | Klik untuk pertanyaan | © Hak Cipta - 2010-2025 : Semua Hak Dilindungi Undang-Undang. | Peta Situs PENCARIAN TERPOPULER Telepon |
| Tel | Nomor telepon 86-18880477902 | Jawa | E-mail |
| Nomor telepon 8618880477902 | Cina | Perancis | |
| Bird | Cina | Perancis | Jerman Spanyol |
| Aquatic animals | Jepang | Korea | Arab Orang yunani |
| Turki | Italia | ||
| Ruminant animal g/head day | January 0.75 | Indonesia Afrikanas Swedia |
Polandia
- Basque
- Catalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgaria
- Bahasa Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroasia
Belanda
| Application object | Bahasa Urdu Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Bahasa Gujarati | Haiti | Bahasa Hausa | Bahasa Kinyarwanda Hmong Hongaria |
| Piglets and fattening pigs | Igbo | Jawa | Bahasa Kannada Khmer Kurdi |
| Kirgistan | Latin | ||
| Bird | 300～400 | 45～60 | orang Makedonia Melayu Bahasa Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwegia
- Bahasa Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbia
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Bahasa Swahili
Tajik
Tamil
Bahasa Telugu
Thai
| Application object | Bahasa Urdu Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Yiddi | Yoruba | Zulu | Bahasa Kinyarwanda Bahasa Oriya Orang Turkmenistan |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
